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Xpression by 400 mM isopropyl-b-D-1-thiogalactopyranoside. The temperature was adjusted to 20 , and bacteria were grown for a further 6 h. Bacterial cells were recovered by centrifugation at 5,000g and frozen as pellets. His-tagged TricSAM was purified by standard immobilized metal-affinity chromatography (GE Healthcare), with the protein recovered by elution with 200 mM imidazole. The elution sam
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Cades. Nematode enzymes mapped to known human disease pathways such as Huntington's disease, Alzheimers disease, Parkinson's disease and Vibrio cholerae infection. The neurological disorder pathways are known to describe the morbidity and depression associated with helminthic infections. The Vibrio cholera infection pathway supports this parasite being similar to gastrointestinal strongylid nemato
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Ange values.Purification of Tric1SAMThe gene fragment corresponding to the SAM domain of At3g49560/Tric1 (amino acids 191?61) was cloned in the pET15b vector (Novagen), yielding an N-terminal His tag fusion protein. Mutations corresponding to the Tric1SAM variants K15A and K20A were introduced by site-directed mutagenesis using the Quikchange II-XL kit (Agilent Technologies). The expression vector
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Those receiving mechanical ventilation during more than 24 h had a mortality of 58.7 , and patients receiving renal replacement therapy had a mortality of 80.0 . Of the patients who died in the hospital, 86.2 were treated with vasopressors, 76.2 received mechanical ventilation during more than 24 hours and 30.0 underwent renal replacement therapy. 50 of patients dying in the ICU died at
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Xpression by 400 mM isopropyl-b-D-1-thiogalactopyranoside. The temperature was adjusted to 20 , and bacteria were grown for a further 6 h. Bacterial cells were recovered by centrifugation at 5,000g and frozen as pellets. His-tagged TricSAM was purified by standard immobilized metal-affinity chromatography (GE Healthcare), with the protein recovered by elution with 200 mM imidazole. The elution sam
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