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Phosphorylation-deficient/mimicking strains had been introduced in the S124 web site (S124A or even S124D) using site-directed mutagenesis (Stratagene) along with normal techniques. Any tetracycline-inducible MDCK variety II mobile or portable line that contain a new Flp Recombinase Focus on (FRT) website in its genome was used because the web host mobile or portable line (Nejsum avec . The new ye
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