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Cence detection.Phase-contrast microscopy Phase-contrast microscopy was performed with a Zeiss Axiovert100 microscope and photomicrographs were taken with an AxioCam MRc5 digital camera.9.10.11.12.Competing interestsThe authors declare that they have no competing interests.13.Authors' contributionsSS participated in the experimental design, implementation and interpretation of results and performe