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Horesis The establishment of the deyolking protocol was a prerequisite for high quality 2D gels from early zebrafish embryos. After removal of the predominant yolk proteins we were able to generate high resolution 2D gels in the acidic (pI 4?, Fig. 4A) as well as in the basic range (pI 6?9, Fig. 4B). We established a protocol that is compatible with three colour fluorescent labelling using the Ett