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Hallenges, particularly concerning respiratory sample collection and transport. Most studies have relied upon clinic or home visits by trained healthcare workers to collect specimens during an ARI episode, which imposes restrictions upon busy families and may lead to biased disease estimates and specimen availability [10-12]. Cost and feasibility of using healthcare workers are also important when
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Hallenges, particularly concerning respiratory sample collection and transport. Most studies have relied upon clinic or home visits by trained healthcare workers to collect specimens during an ARI episode, which imposes restrictions upon busy families and may lead to biased disease estimates and specimen availability [10-12]. Cost and feasibility of using healthcare workers are also important when
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
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He first year mould was seen in some samples as they arrived in the laboratory and we became concerned about the impact of this contaminant upon sample integrity. Therefore, as part of the ORChID study, we undertook a broader investigation of sample quality, examining collection and transportation, and how these impact on respiratory virus detection. Our objectives were first to determine the qual
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S Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Alsaleh et al. BMC Infectious Diseases 2014, 14:15 http://www.biomedcentral.com/1471-2334/14/Page 2 ofBackground Acute respiratory infections (ARIs) caused by viruses are the most common illnesses experienced