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Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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T ambient temperature has limited or no impact on respiratory virus detection by PCR [14,20,21], although investigating further the effects of transporting samples for extended periods and at higher temperatures was highlighted in one study [20]. The observational research in childhood infectious diseases (ORChID) project is a longitudinal, communitybased, dynamic birth cohort study, which seeks t
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He first year mould was seen in some samples as they arrived in the laboratory and we became concerned about the impact of this contaminant upon sample integrity. Therefore, as part of the ORChID study, we undertook a broader investigation of sample quality, examining collection and transportation, and how these impact on respiratory virus detection. Our objectives were first to determine the qual