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Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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Ecting a healthy term baby were recruited antenatally at either the publically funded Royal Brisbane and Women's Hospital or the North West Private Hospital, in Brisbane, Australia, a subtropical city of more than 2 million inhabitants [22].Ethics statementThe Human Research Ethics Committees of the Children's Health Queensland Hospital and Health Service, the Royal Brisbane and Women's Hospital a
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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T tube that contained a viral transport media-soaked foam pad in the base. Parents were instructed to squeeze the foam pad to release the fluid and bathe the top of the swab. Ideally within 24hours of collection, the nasal swabs were then sent byAlsaleh et al. BMC Infectious Diseases 2014, 14:15 http://www.biomedcentral.com/1471-2334/14/Page 3 ofregular postal mail (in accordance with Australia Po
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Ecting a healthy term baby were recruited antenatally at either the publically funded Royal Brisbane and Women's Hospital or the North West Private Hospital, in Brisbane, Australia, a subtropical city of more than 2 million inhabitants [22].Ethics statementThe Human Research Ethics Committees of the Children's Health Queensland Hospital and Health Service, the Royal Brisbane and Women's Hospital a
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He first year mould was seen in some samples as they arrived in the laboratory and we became concerned about the impact of this contaminant upon sample integrity. Therefore, as part of the ORChID study, we undertook a broader investigation of sample quality, examining collection and transportation, and how these impact on respiratory virus detection. Our objectives were first to determine the qual