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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
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Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
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Hallenges, particularly concerning respiratory sample collection and transport. Most studies have relied upon clinic or home visits by trained healthcare workers to collect specimens during an ARI episode, which imposes restrictions upon busy families and may lead to biased disease estimates and specimen availability [10-12]. Cost and feasibility of using healthcare workers are also important when
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Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
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Eir infant's second birthday. Instructions on sample collection were provided at the initial visit by research staff who also demonstrated the technique by undertaking the initial nasal swab specimen shortly after delivery of the newborn baby. In addition, parents were given written instructions on how to collect nasal swab specimens. They also received regular text messages, emails or telephone c
1
Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
1
Eir infant's second birthday. Instructions on sample collection were provided at the initial visit by research staff who also demonstrated the technique by undertaking the initial nasal swab specimen shortly after delivery of the newborn baby. In addition, parents were given written instructions on how to collect nasal swab specimens. They also received regular text messages, emails or telephone c