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T ambient temperature has limited or no impact on respiratory virus detection by PCR [14,20,21], although investigating further the effects of transporting samples for extended periods and at higher temperatures was highlighted in one study [20]. The observational research in childhood infectious diseases (ORChID) project is a longitudinal, communitybased, dynamic birth cohort study, which seeks t
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He first year mould was seen in some samples as they arrived in the laboratory and we became concerned about the impact of this contaminant upon sample integrity. Therefore, as part of the ORChID study, we undertook a broader investigation of sample quality, examining collection and transportation, and how these impact on respiratory virus detection. Our objectives were first to determine the qual
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Hallenges, particularly concerning respiratory sample collection and transport. Most studies have relied upon clinic or home visits by trained healthcare workers to collect specimens during an ARI episode, which imposes restrictions upon busy families and may lead to biased disease estimates and specimen availability [10-12]. Cost and feasibility of using healthcare workers are also important when
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Hallenges, particularly concerning respiratory sample collection and transport. Most studies have relied upon clinic or home visits by trained healthcare workers to collect specimens during an ARI episode, which imposes restrictions upon busy families and may lead to biased disease estimates and specimen availability [10-12]. Cost and feasibility of using healthcare workers are also important when
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Eir infant's second birthday. Instructions on sample collection were provided at the initial visit by research staff who also demonstrated the technique by undertaking the initial nasal swab specimen shortly after delivery of the newborn baby. In addition, parents were given written instructions on how to collect nasal swab specimens. They also received regular text messages, emails or telephone c
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T ambient temperature has limited or no impact on respiratory virus detection by PCR [14,20,21], although investigating further the effects of transporting samples for extended periods and at higher temperatures was highlighted in one study [20]. The observational research in childhood infectious diseases (ORChID) project is a longitudinal, communitybased, dynamic birth cohort study, which seeks t
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Ecting a healthy term baby were recruited antenatally at either the publically funded Royal Brisbane and Women's Hospital or the North West Private Hospital, in Brisbane, Australia, a subtropical city of more than 2 million inhabitants [22].Ethics statementThe Human Research Ethics Committees of the Children's Health Queensland Hospital and Health Service, the Royal Brisbane and Women's Hospital a
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Hallenges, particularly concerning respiratory sample collection and transport. Most studies have relied upon clinic or home visits by trained healthcare workers to collect specimens during an ARI episode, which imposes restrictions upon busy families and may lead to biased disease estimates and specimen availability [10-12]. Cost and feasibility of using healthcare workers are also important when